Fig. 2: Enhanced RNAi efficiency in S. frugiperda by dsRNA@ZIF-8@PDA.
a Phenotype of body size and b gut tissue of the S. frugiperda larvae treated with naked dsRNA (dsGFP, dsCHS, and dsV-ATPaseB), ZIF-8@PDA, dsCHS@ZIF-8@PDA, and dsV-ATPaseB@ZIF-8@PDA after 4 days. c The survival rate (%) of S. frugiperda 3rd-instar larvae with different treatments within 4 days. Kaplan-Meier survival analysis with log-rank test was used to assess differences (n = 100/ group, The asterisk indicates significance: *P < 0.05, **P < 0.01, and ***P < 0.001). d body weight, e body length, and f accumulated frass weight of the S. frugiperda larvae under different treatments after 4 days. (Mean ± SD; Body length: n = 36/group, Body weight: n = 36/group, Frass mass: n = 10/group; Different letters above each bar indicate significant differences at P < 0.05 as determined by one-way ANOVA with Tukey HSD test; Body length: F5,215 = 724.774, P < 0.001; Body weight: F5,215 = 508.014, P < 0.001; Frass mass: F5,59 = 300.678, P < 0.001). Exact P-values are reported in the source data. g, h Knockdown efficiency of dsCHS and dsV-ATPaseB of S. frugiperda after feeding on naked dsRNA (dsGFP, dsCHS, and dsV-ATPaseB), ZIF-8@PDA, dsCHS@ZIF-8@PDA, and dsV-ATPaseB@ZIF-8@PDA after 4th day, respectively. (Mean ± SD; n = 4 replicates, 3 larvae/replicates; V-ATPaseB: F3,15 = 94.34, P < 0.001; CHS: F3,15 = 185.117, P < 0.001). Exact P-values are reported in the source data. Relevant source data are provided as a source data file.
