Fig. 5: 18F-FDS uptake by clinically relevant molds. | Nature Communications

Fig. 5: 18F-FDS uptake by clinically relevant molds.

From: 18F-Fluorodeoxysorbitol PET for noninvasive detection of invasive mold infections: preclinical and first-in-human studies

Fig. 5: 18F-FDS uptake by clinically relevant molds.The alt text for this image may have been generated using AI.

a Methodology for 18F-FDS in vitro uptake. Briefly, molds were co-incubated with 18F-FDS for two hours, centrifuged to form a pellet and then washed. 18F-FDS uptake in the pellet was measured as becquerel (Bq) and corrected for protein (mg). Heat killed (HK) molds were generated by exposing them to 95 °C for 60 min. b–d 18F-FDS uptake by live and HK Aspergillus fumigatus (ATCC 1022) (red), Rhizopus arrhizus (green), and Mucor circinelloides (blue). The x-axis (time) in panel b is in min. e–g Competitive inhibition of 18F-FDS uptake with increasing concentrations of cold (unlabeled) sorbitol in Aspergillus (red), Rhizopus (green), and Mucor (blue). h 18F-FDS uptake (at 120 min) by the reference A. fumigatus (ATCC 1022) and 30 randomly selected clinical isolates representing a range of clinically relevant molds including Lichtheimia corymbifera, Syncephalastrum racemosum, Cunninghamella bertholletiae, Cladophialophora bantiana (clinical isolate from patient 4), and Fusarium solani. All assays were performed in triplicate. Data are shown as median ± IQR. Panel (a) created using BioRender. Masias, Y. (2025) https://BioRender.com/w9dq20g.

Back to article page