Fig. 1: Defects of the gip1 mutant in sexual development.

a Mating cultures of the wild type (WT) and gip1 mutant were examined for perithecium formation on carrot agar (CA) mating plates and wheat straws (WS) at 7 days post-fertilization (dpf). Bar = 0.5 mm. b SEM examination of fruiting bodies formed by the WT and gip1 mutant on wheat straws at the marked time points. Bar = 20 μm. c A close-up examination of sexual fruiting bodies formed by the WT, gip1 mutant, and mat1-1-1 mutant on wheat straws at 7-dpf. Bar = 20 μm. d Thick sections of sexual fruiting bodies formed by the same set of strains. P peridium, A ascogenous tissue. Bar = 20 μm. e The cross between the gip1 mutant (female) and the H1-GFP transformant of the wild type (male) resulted in fertile perithecia (Per) and asci containing 8 ascospores. Epifluorescence microscopical examination of 100 fertile perithecia showed the 1:1 segregation of H1-GFP signals in the nucleus. Bar = 0.5 mm for examination of perithecia, and bar = 20 μm for asci/ascospores. For a–e, similar results were obtained in three independent experiments.