Fig. 5: The isolation and subsequent characterization of spontaneous suppressors of the gip1 mutant. | Nature Communications

Fig. 5: The isolation and subsequent characterization of spontaneous suppressors of the gip1 mutant.

From: Gip1 GPCR regulates two sexual-stage differentiation processes in the ascomycete Fusarium graminearum

Fig. 5: The isolation and subsequent characterization of spontaneous suppressors of the gip1 mutant.

a CA mating cultures (upper panels, bar = 0.5 mm) of the gip1 mutant with or without clusters of perithecia (marked with arrow) that contained asci and ascospores (lower panels, bar = 50 μm). b Three-day-old PDA cultures of the wild type (WT), gip1 mutant, and ten spontaneous suppressor strains. c CA mating cultures of suppressor strains were examined for perithecium/PIs formation (upper panels, bar = 0.5 mm) and asci/ascospores (lower panels, bar = 50 μm) at 14-dpf. d SEM examination (upper panels) and thick sections (lower panels) of perithecia formed by the WT and PIs formed by suppressor S1 on CA plates at 7-dpf. Bar = 20 μm. e Flowering wheat heads drop-inoculated with conidia from the WT, gip1 mutant, and ten spontaneous suppressor strains at 14-dpi. The inoculation sites were indicated with black dots. For a–d, similar results were obtained in three independent experiments.

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