Fig. 6: Suppressive mutations in FgVeA.
a Spontaneous suppressor mutations identified in FgVeA and sequence alignment of the Velvet domain (purple box) of FgVeA and its orthologs from labeled ascomycetes. PEST, proline-, glutamate-, serine-, and threonine-rich motif. b Cultures of three selected spontaneous suppressor strains (S1, S2, and S7), FgVeAS479fs, FgVeAP39L, FgVeAG199S, gip1 FgVeAS479fs, gip1 FgVeAP39L, and gip1 FgVeAG199S mutants were examined for colony growth on PDA (left panels) and perithecia/PIs formation on CA plates (right panels). Bar = 0.5 mm. Similar results were obtained in three independent experiments. Whereas suppressor S1 and gip1 FgVeAS479fs transformants only produced PIs, all other strains produced fertile perithecia with ascospore cirrhi. c The tertiary structure of the FgVeA (gray) and FgVelB (green) dimer predicted with AlphaFold v2 and visualized with the UCSF Chimera tool. The six mutation sites in the Velvet domain (blue) are in red. d The relative expression levels of five selected down-regulated genes assayed by qRT-PCR with RNA isolated from mating cultures of the indicated strains sampled at 3-dpf. Mean and SD were estimated with data from three independent replicates (n = 3). Source data are provided as a Source Data file.
