Fig. 4: Cryo-EM structure of USP1/UAF1 bound to K63-UbVAUb.
A Domain architecture of USP1 and UAF1. Density was resolved for USP1’s insert 3 (dark gray), but not for insert 1 and insert 2 (light gray). B Final map of 3.0 Å final structure. DeepEMhancer sharpened map is colored as in A and shown at threshold 0.005. C DeepEMhancer sharpened map from (B), placed in the non-sharpened final density map (gray transparent map) shown at threshold 0.015 to accentuate density for flexible regions. D Atomic reconstruction of the structure of USP1/UAF1 bound to K63-linked UbVAUb. E Zoom showing the backbone of insert 3 of USP1 in the non-sharpened map shows how it anchors at the WD40 domain of UAF1.
