Fig. 1: Global RIPK4 deficiency in adult mice induces osteoporosis and myeloid-biased hematopoiesis.

a–c Representative μCT reconstructive images of 16-week-old male Ripk4^fl/fl and Ubc^Cre/ERT2 Ripk4^fl/fl mice femur (a) and trabecular (BMD, BV/TV, Tb.N, Tb.Sp and Tb.Th) (b) and cortical microstructural parameters (Ct.Th and Ct.Po) (c) derived from μCT analysis, demonstrating obvious bone loss in Ubc^Cre/ERT2 Ripk4^fl/fl Rhot1^fl/fl mice (n = 4 per group). d, e Representative μCT reconstructive images of 16-week-old male male Ripk4^fl/fl and Ubc^Cre/ERT2 Ripk4^fl/fl mice spine (d) and trabecular microstructural parameters (e) derived from μCT analysis (n = 4 per group). f, g Representative image of flow cytometry (f) and analysis of proportions of myeloid progenitors (CMP, GMP and MEP) (g) (n = 5 in Ripk4^fl/fl group and n = 6 in Ubc^Cre/ERT2 Ripk4^fl/fl group). h, i Representative image of flow cytometry (h) and analysis of proportions of CD11b⁺ myeloid cells (i) of 16-week-old Ripk4^fl/fl and Ubc^Cre/ERT2 Ripk4^fl/fl mice (n = 5 in Ripk4^fl/fl group and n = 6 in Ubc^Cre/ERT2 Ripk4^fl/fl group). j, k Representative image of flow cytometry (j) and analysis of proportions of B220⁺ lymphoid cells (k) of 16-week-old Ripk4^fl/fl and Ubc^Cre/ERT2 Ripk4^fl/fl mice (n = 5 in Ripk4^fl/fl group and n = 6 in Ubc^Cre/ERT2 Ripk4^fl/fl group). l, m Representative image of flow cytometry (l) and analysis of proportions of monocyte, Ly-6C^int and Ly-6C^hi monocytes (m) of 16-week-old Ripk4^fl/fl and Ubc^Cre/ERT2 Ripk4^fl/fl mice (n = 5 in Ripk4^fl/fl group and n = 6 in Ubc^Cre/ERT2 Ripk4^fl/fl group). Data are presented as mean ± s.d., with biologically individual data points shown. P values were determined by unpaired two-tailed Student’s t-test (BV/TV, Tb.N, Tb.Sp, Tb.Th of b, c, e, i, k, m), unpaired two-tailed Student’s t test with Welch’s correction (g), and two-tailed Mann-Whitney U test (BMD of b). Source data are provided as a Source Data file.