Fig. 3: Osteolineage RIPK4 deficiency impairs osteogenesis. | Nature Communications

Fig. 3: Osteolineage RIPK4 deficiency impairs osteogenesis.

From: RIPK4-mediated MFN2 degradation drives osteogenesis through mitochondrial fragmentation and restricts myelopoiesis by blocking mitochondrial transfer

Fig. 3: Osteolineage RIPK4 deficiency impairs osteogenesis.

a–c Representative μCT reconstructive images of male Ripk4fl/fl and Prrx1Cre Ripk4fl/fl mice femur at 16 weeks (a) and trabecular microstructural parameters (BMD, BV/TV, Tb.N, Tb.Sp and Tb.Th) (b) and cortical microstructural parameters (Ct.Th and Ct.Po) (c) derived from μCT analysis (n = 6 per group), demonstrating significant bone loss after osteolineage RIPK4 ablation. d–f Goldner trichrome staining of male Ripk4fl/fl and Prrx1Cre Ripk4fl/fl mice femur at 16 weeks (d) and histomorphometry analysis of BV/TV, Tb.N (e), N.Ob/BS and OS/BS (f) (n = 3 per group). Scale bar, 20 µm. g, h Representative images of calcein double labeling of the mineral layers of male Ripk4fl/fl and Prrx1Cre Ripk4fl/fl mice femur at 16 weeks (g) and histomorphometry analysis of the BFR/BS, MAR, and MS/BS (h) (n = 4 per group). Scale bar, 50 µm. i, j ALP staining (i) and alizarin red staining (j) of in vitro osteogenesis from Ripk4fl/fl and Prrx1Cre Ripk4fl/fl mice. Scale bar, 250 µm. k qPCR analysis of the expression of osteoblast signature genes at the mRNA level between Ripk4fl/fl and Prrx1Cre Ripk4fl/fl mice (n = 3 per group). Data are presented as mean ± s.d., with biologically individual data points shown. P values were determined by unpaired two-tailed Student’s t-test (BMD, BV/TV, Tb.N, Tb.Sp of (b, c), BV/TV of (e, f), MAR of (h), Alpl, Bglap, Col1a1, Sp7, Runx2 of (k)), unpaired two-tailed Student’s t test with Welch’s correction (Tb.N of (e), MS/BS, BFR/BS of (h)), and Mann–Whitney U test (Tb.Th of (b), Spp1 of (k)). Source data are provided as a Source Data file.

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