Fig. 4: Osteolineage RIPK4 deficiency results in myeloid-biased hematopoiesis. | Nature Communications

Fig. 4: Osteolineage RIPK4 deficiency results in myeloid-biased hematopoiesis.

From: RIPK4-mediated MFN2 degradation drives osteogenesis through mitochondrial fragmentation and restricts myelopoiesis by blocking mitochondrial transfer

Fig. 4: Osteolineage RIPK4 deficiency results in myeloid-biased hematopoiesis.

a Flow cytometry analysis of proportions of LT-HSC and ST-HSC in 16-week-old male Ripk4fl/fl and Prrx1Cre Ripk4fl/fl mice femur (n = 3 per group). b Flow cytometry analysis of proportions of CMP, GMP, and MEP in 16-week-old male Ripk4fl/fl and Prrx1Cre Ripk4fl/fl mice femur (n = 3 per group). c Flow cytometry analysis of proportions of CD11b+ myeloid cells, monocytes and B220+ lymphoid cells in male Ripk4fl/fl and Prrx1Cre Ripk4fl/fl mice femur (n = 3 per group). d Flow cytometry analysis of proportions of LT-HSC and ST-HSC in16-week-old male Ripk4fl/fl and Dmp1Cre Ripk4fl/fl mice femur (n = 5 per group). e Flow cytometry analysis of proportions of CMP, GMP and MEP in16-week-old male Ripk4fl/fl and Dmp1Cre Ripk4fl/fl mice femur (n = 5 per group). f Flow cytometry analysis of proportions of CD11b+ myeloid cells, monocytes and B220+ lymphoid cells in 16-week-old male Ripk4fl/fl and Dmp1Cre Ripk4fl/fl mice femur (n = 5 per group). g Flow cytometry analysis of proportions of CD11b+ myeloid cells, monocytes, neutrophils and B220+ lymphoid cells in16-week-old male Ripk4fl/fl and Lyz2Cre Ripk4fl/fl mice femur (n = 3 per group). h Flow cytometry analysis of proportions of CD11b+ myeloid cells, monocytes, neutrophils and B220+ lymphoid cells in 16-week-old male Ripk4fl/fl and Cd4Cre Ripk4fl/fl mice femur s (n = 4 in Ripk4fl/fl group and n = 3 in Cd4Cre Ripk4fl/fl group). Data are presented as mean ± s.d., with biologically individual data points shown. P values were determined by unpaired two-tailed Student’s t-test (a, b, c, CMP, MEP of e, f, g), unpaired two-tailed Student’s t test with Welch’s correction (GMP of e, h), and Mann-Whitney U test (d, g). Source data are provided as a Source Data file.

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