Fig. 2: HCMV cellular remodeling, vIAC formation, and progeny virion composition are dependent on macroH2A1.
From: Human cytomegalovirus induces neuronal gene expression through IE1 for viral maturation
A Representative transmission electron microscopy (TEM) images of mock-treated WT and macroH2A1 KO HFF-T cells showing the uninfected state of the endoplasmic reticulum (ER). B Representative TEM images at 4 days post infection (dpi) with HCMV. C Representative TEM images of viral-induced assembly compartments (vIAC) at 4dpi as indicated. Below: Zooms with annotated versions to the right. Scale bars as indicated. D Quantification of vIAC subcompartment area. Violin plot depicts median, and upper, and lower quartiles as dotted lines. *** denotes p < 0.001 or p = 4.3×10−4 by Kolmogorov-Smirnov test with n = 143 for WT and n = 138 for KO. E Scatter plot of HCMV viral proteins identified by LC-MS in virion preparations from WT- and macroH2A1 KO-infected cells. Enriched proteins (fold change [FC] ≥ 1) in macroH2A1 KO virion preparations are shown as gray dots, while depleted proteins (FC ≤ −1) are highlighted in orange (n = 3 technical replicates), envelope proteins in red. The identified HCMV viral proteome was normalized to the major capsid protein (MCP, UL86), which exhibited consistent abundance across all conditions. See Sup Fig. 3 and Sup Data 1 for further details.
