Fig. 3: Host gene expression is altered upon loss of macroH2A1 during HCMV infection.
From: Human cytomegalovirus induces neuronal gene expression through IE1 for viral maturation
A K-means clustering (k = 4) of gene expression changes over 72 hours of infection shown as a heatmap. Z-scores were calculated for each gene from its normalized count across the time course of CMV infection for WT and macroH2A1 KO cells. B The -log10(FDR) value for enrichment of neuronal GO categories in Cluster 1. C Volcano plot where the Log2(Fold Change) for WT vs. macroH2A1 KO is plotted against -log10(FDR) for genes in Cluster 1. Genes with Log2(Fold Change) > 1 and FDR ≤ 0.05 are marked in red. Neuronal genes selected for further characterization are labeled in blue. D Matrix of Euclidean distance between normalized expression profiles of CMV infection time course for WT and macroH2A1 KO, and other cell types. Gene expression datasets for other cell types were obtained from ENCODE. E PCA plot showing PC1 and PC3 for the same expression profiles plotted in (D).
