Fig. 1: The workflow and host details of gut phage isolates.
From: Gut Phage Biobank: a collection of bacteriophages targeting human commensal bacteria
A The comprehensive workflow for efficient gut phage isolation. The classic method involves a single co-culture of the bacterial host with the source sample, followed by concentration for plaque assays. The repetitive enrichment method enhances this approach by introducing multiple rounds of co-culture to increase the abundance of targeting phages. The metagenomics-guided method further refines the process by incorporating a metagenomic analysis step before selecting samples for repetitive co-culture and concentration. The numbers below each method indicate the number of phage isolates obtained and the corresponding number of infected bacterial hosts. B The three-column Sankey diagram visualizes the host species of GPB phages (middle column), whether these hosts are abundant within the human gut (left column), and the types of diseases associated with these host bacteria (right column). The Venn diagram illustrates the number of phages targeting highly abundant gut bacteria, disease-related gut bacteria, and their overlap.
