Fig. 3: Discovery and characterization of MxaF/MxaJ complex.
From: Deciphering the assembly process of PQQ dependent methanol dehydrogenase
a Kinetic parameter of MDHs extracted from different strains. The preparations been treated with 10 mM Ca2+ in vitro at pH 9.5 were highlighted in bold and orange. Data are represented as mean ± SD. b Native-PAGE analysis of the aggregates in the purified MDH preparations from different strains (One representative figure is shown in 3 independent experiments). Protein MxaJ was set as a control. c Native-PAGE analysis of the aggregates in MDHs purified from E. coli strains containing 1 (E. coli-9) or 2 (E. coli-10) mxaI genes under the gradient (0, 10, 20 μM) PQQ supply. d Specific activities of the MDHs in c after Ca2+ treatment in vitro. e Native-PAGE analysis of the aggregates in different assembly reactions in vitro. f Specific activities of the mixtures in e after been treated with 10 mM CaCl2 in vitro at pH 9.5. The error bars represent the standard deviation from the mean, originating from four and three independent activity analyses, respectively, of the samples in c and e. P values were determined using a two-sided unpaired t-test.
