Fig. 2: Sustained intracellular myelin accumulation increases UBE3A in macrophages. | Nature Communications

Fig. 2: Sustained intracellular myelin accumulation increases UBE3A in macrophages.

From: UBE3A promotes foam cell formation and counters remyelination by targeting ABCA1 for proteasomal degradation

Fig. 2: Sustained intracellular myelin accumulation increases UBE3A in macrophages.The alternative text for this image may have been generated using AI.

A,B Ube3a mRNA (A, n = 3 samples) and UBE3A protein level (B, n = 6 samples) in mouse bone marrow-derived macrophages (BMDMs) treated with myelin (50 µg/ml) for 24 h (Mye24) or 72 h (Mye72), and normalized to untreated cells (Ctrl, dotted line). C UBE3A mRNA in human monocyte-derived macrophages treated with myelin (50 µg/ml) for 24 h (Mye24) or 72 h (Mye72), and normalized to untreated cells (dotted line) (n = 3 samples). D,E,H Representative images (G) and quantification (D, E) of active MS lesion stained for UBE3A/CD68. Scale bars 300 and 50 µm. [n(NAWM) = 4 subjects, n(Rim) = 4 subjects, n(center) = 8 subjects]. F, I Representative images (I) and quantification (F) of active MS lesion stained for ABCA1/HLA-DR/UBE3A. Scale bar 50 µm (n = 4 subjects). Data were representative of ≥3 independent experiments and are shown as mean ± SEM. Statistical significance was determined by two-sided Student’s t-test (A–C) or two-sided one-way ANOVA (D–F). *P < 0.05, **P < 0.01, and ***P < 0.001. If p values are not indicated, the result was not significant.

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