Fig. 9: Ube3a-deficiency stimulates remyelination in the cuprizone model.

Wildtype (Wt) and Ube3a^−/− mice were fed a cuprizone diet for 5 weeks to induce demyelination in the corpus callosum (cc). Upon withdrawal of the cuprizone diet, spontaneous remyelination ensues. Tissue was collected after demyelination (5w) and after 1 week of recovery (5 + 1w). A, B Representative pictures and quantification of F4/80 PLIN2 co-staining of cc. Scale bar 100 µm. [n(Wt, 5w) = 6 mice, n(Ube3a^−/−,5w) = 5 mice, n(Wt, 6w) = 5 mice, n(Ube3a^−/−,6w) = 4 mice]. C ABCA1 protein level in cc determined with immunoblotting and normalized to Cy5 levels (loading control). [n(Wt) = 3 mice, n(Ube3a^−/−) = 4 mice]. D Gene expression of pro-inflammatory genes Nos2, Il6, and Ccl4 in cc. [n(Wt, 5w) = 6 mice, n(Ube3a^−/−,5w) = 3 mice, n(Wt, 6w) = 6 mice, n(Ube3a^−/−,6w) = 4 mice]. E, F Representative pictures (E) and quantification (F) of myelin basic protein (MBP) staining of the cc. Scale bar 100 µm. [n(Wt, 5w) = 6 mice, n(Ube3a^−/−,5w) =  3 mice, n(Wt, 6w) = 6 mice, n(Ube3a^−/−,6w) = 4 mice]. All data were represented as mean ± SEM. Statistical significance was determined by two-sided one-way ANOVA (B, C, F) or two-sided Student’s t-test (D). *P < 0.05 and **P < 0.01. If p values are not indicated, the result was not significant.