Fig. 1: Inositol depletion impedes root growth and reveals cell plate developmental defects. | Nature Communications

Fig. 1: Inositol depletion impedes root growth and reveals cell plate developmental defects.

From: Phosphatidylinositides regulate the cell plate morphology transition during cytokinesis in Arabidopsis

Fig. 1

a Representative images of 5 DAG wild type and mips1 mips3 seedlings grown without or with 0.1 or 0.5 g/L inositol. Scale bar: 5 mm. b Quantification of root length under the same conditions. Data were analyzed using one-way ANOVA with multiple comparisons (wild type: -inositol, n = 22; 0.1 g/L inositol, n = 28; 0.5 g/L inositol, n = 35. mips1 mips3: -inositol, n = 28; 0.1 g/L inositol, n = 25; 0.5 g/L inositol, n = 29). n.s. P = 0.9676; *** P < 0.0001. c Representative 3D projection images of HTR12-GFP-labeled root epidermal cells (10 DAG). Dots represent centromeres. Red asterisk indicates polyploid macronuclei. Scale bars: 5 μm. d Quantification of endoreduplication for each genotype (n = 5 roots, >50 cells analyzed in total). Two-tailed Student’s t-test, *** P < 0.0001. e FM 4-64-stained root epidermal cells show incomplete cell walls (white arrows) and thickened stubs (white arrowheads) in mips1 mips3 grown without inositol. Scale bars: 10 μm. The experiment was repeated more than three times with similar results. f 3D projections of cell plates (top) and cell walls (bottom). Black arrow indicates donut-shaped cell plate in mips1 mips3; white arrow points to a hole between neighboring cells. Scale bars: 5 μm. g Representative TEM images of dividing root epidermal cells at the disk and discontinuous phragmoplast stages. Red arrows show swollen, incomplete cell plates with gaps; red arrowheads highlight unfused vesicles. n: nucleus. Scale bars: 0.5 μm or 1 μm. h Quantification of cell plate thickness as shown in (g) (wild type, n = 76; mips1 mips3, n = 73) from >10 cells per genotype. For each cell plate, thickness was measured at 5 to 6 evenly spaced positions along the lagging zone. Two-tailed Student’s t-test, *** P < 0.0001. Data in (b, d, h) are shown as box and whisker plots with individual points, representing three experimental replications with similar results. Source data are provided as a Source Data file.

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