Fig. 7: Co-assembly of nanosensor/cell hybrid arrays for metabolite profiling and drug response assay. | Nature Communications

Fig. 7: Co-assembly of nanosensor/cell hybrid arrays for metabolite profiling and drug response assay.

From: Massively parallel microbubble nano-assembly

Fig. 7

Nanosensors: Ag-coated nanospheres; cells: Gram-positive B. Subtilis. Controlled cell counts in the hybrid arrays are determined by bubble size, ~98 µm for (a) and ~54 µm for (b). Continuous metabolite profiling up to 3 h in response to vancomycin for (c) large clusters in (a), and d small clusters in (b), exhibits (e) distinct diminishing time depending on average cell counts analyzed with Mann–Whitney U Test. Island-large clusters: Z value = −1.98; Large clusters-small clusters: Z value = −2.78; Islands-small clusters: Z value = −2.72. Raman signals in green and red for metabolites and the nanosphere sensors, respectively (c) d shows 4 representative measurement results. Additional data for (c, d) are available in Fig. S14. Scale bars: (a) 5 μm and (b) 2 μm. Error bars: Standard deviation (s.d.). Source data are provided as a Source Data file.

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