Fig. 3: The H4c residues K107 and R112 are essential for the regulation of NaCT by SLC26A1 and the control of cellular metabolism.
A The structure of human NaCT shows H4c, H6b, and HPin. The K107 and R112 residues are highlighted within the H4c domain (inset). B Summary and C Traces of NaCT currents measured in Xenopus oocytes injected with NaCT-WT, K107A, R112A, F128A (dysfunctional), or SLC26A1, as indicated. N represents the current measurement in a single oocyte. D Representative traces and E Summary of NaCT function in mammalian HEK293-T cells transfected with NaCT(WT), K107A, R112A, or SLC26A1 (as in B and C). Real-time fluorescent measurements were performed in cells loaded with the ING-2 Na+-sensitive dye using the protocol described in the figure and the methods section. N represents the average changes in fluorescence relative to NaCT(WT) in the cells that were cultured on a single cover slip. F Glycostress protocol performed using HEK293-T cell cultures expressing either control, WT(NaCT), K107A, or R112A constructs. Statistical analysis of the seahorse measurements is in Supplementary Fig. 5B–E. Data are presented as mean values +\− SEM. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparison test.
