Fig. 5: The NaCT K107 mediates interactions with H4c and H6b residues to control NaCT function, regulation by partner proteins, and the glycolytic flux.
A The K107-E305-K308 triad (orange) is highlighted in the NaCT structure (H4c-light blue, H6b-cyan, HPin-green). B Summary and C representative traces of NaCT currents in Xenopus oocytes expressing different K107, E305, and K308 mutants. D Representative WB blot analysis of a CoIP assay performed in HEK293-T cells transfected with NaCT WT, SLC26A6 WT (A6), and NaCT mutants, as indicated. The red arrow indicates the size of SLC26A6 in the coIP and blot. Densitometry and statistical analysis are shown in Supplementary Fig. 11. E Schematic summary of different K107-E305-K308 triad mutations and their functional outcome (from B and C). Data are presented as mean values +\− SEM. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparison test.
