Fig. 1: In vitro cleavage activities of NsCas9d and the protospacer adjacent motif (PAM) identification.
From: Structural insights into Type II-D Cas9 and its robust cleavage activity
a Phylogenetic representation of the diversity provided by Cas9 orthologs. Subtype II-A, B, C, D systems are colored green, purple, orange and red respectively. Several typical Cas9s are indicated by black dots. b In vitro cleavage activity of NsCas9d. dsDNA substrates of varying base pairing with guide of sgRNA were used to explore the minimal heteroduplex length requisite for sustained high cleavage activity. c Depletion assay to identify functional PAM for NsCas9d. Log relative frequency of each base at every position relative to 3′ end of target sequence from libraries before and after cleavage. d In vitro cleavage activity of NsCas9d. The dsDNA targets have different sequences at the PAM region. e In vitro cleavage activity of several Cas9 proteins. Source data are provided as a Source Data file.
