Fig. 2: Structure of NsCas9d-sgRNA-dsDNA ternary.
From: Structural insights into Type II-D Cas9 and its robust cleavage activity
a Architectural domains of NsCas9d. BH, bridge helix. WED wedge, PI PAM-interacting. b Schematic representation of the sgRNA and dsDNA used for cryo-EM. TS, target strand. NTS non-target strand. c Cartoon representations of NsCas9d-sgRNA-dsDNA complex in a catalytic state. Domains of NsCas9d are colored according to the scheme used in (a). d Overall structure of NsCas9d-sgRNA-dsDNA complex, while NsCas9d in surface style and nucleic acid in cartoon style.
