Fig. 3: Structural features of sgRNA.
From: Structural insights into Type II-D Cas9 and its robust cleavage activity
a Schematics of the sgRNA depicting secondary structure. b The interactions of N659 and upstream portion of the repeat: anti-repeat duplex. c In vitro cleavage activity of NsCas9d with sgRNAs which have different length of repeat: anti-repeat duplex via truncation. Source data are provided as a Source Data file. d, e Cartoon representation of the tracrRNA scaffold of NsCas9d and the ωRNA of IscB (PDB: 7UTN), colored according to the scheme used in (a). f The interaction of sgRNA with BH and the loop connecting two REC domains. The protein part is represented in the form of surface electrostatic potential. g The stacking interactions of the flip within sgRNA and residues of NsCas9d. h Cartoon representation of the pseudoknot structure.
