Fig. 4: PAM recognition of NsCas9d.
From: Structural insights into Type II-D Cas9 and its robust cleavage activity
a Binding of the PAM duplex with NsCas9d. NsCas9d was shown in cartoon representation with the coloring of the domains and residues referenced from Fig. 2a. b Interactions of residues and the PAM duplex. Residues are color-coded by domains in Fig. 2a. c Effects on in vitro cleavage of mutations on the PAM-interacting residues. Data are represented as mean ± SD (n = 3 independent experiments). Source data are provided as a Source Data file.
