Fig. 4: Epiregulon uncovers context-dependent interaction partners of SMARCA4.

a Immunoblotting of SMARCA4 after 24 h of treatment and HDAC as a loading control. b SMARCA4 activity computed by Epiregulon for all 6 prostate cell lines after 24 h of treatment. Boxplots presented as median values ± 25%. Lower whisker is the smallest observation ≥25% quantile −1.5 × interquantile range (IQR). Upper whisker represents the largest observation ≤75% + 1.5 × IQR. c Altered regulon size indicates the number of altered target genes mapped to each regulator. Altered genes are defined by genes with logFC > 0.5 and FDR < 0.05 after SMARCA2_4.1 treatment. LogFC in activity indicates the changes in regulator activity estimated by Epiregulon. d ChIP-seq of AR, SMARCA4 and FOXA1 in MDA-PCa-2b treated with the SMARCA2/4 degrader, SMARCA2_4.1 for 24 h at 0.1 µM at SMARCA4 binding sites. e Representative regions of SMARCA4, AR and FOXA1 ChIP-seq in MDA-PCa-2b cells treated with SMARCA2_4.1. f Same as c, but for DU145 cells. g ChIP-seq of SMARCA4, TEAD1 and FOSL1 in DU145 cells treated with the SMARCA2/4 degrader, SMARCA2_4.1 for 24 h at 0.1 µM. h Representative regions of SMARCA4, TEAD1 and FOSL1 ChIP-seq in DU145 cells treated with SMARCA2_4.1. Created in BioRender. Yao, X. (2025) https://BioRender.com/x50fdft.