Fig. 4: OsBZR4 suppresses YUC4 and PIN5b expression.
From: OsBZR4 regulates temperature-dependent embryogenesis in rice
a Relative expression of YUC genes in 3-DAP caryopses. n = 3 biological replicates. b EMSA confirming that the His-OsBZR4 protein binds to the YUC4 promoter region containing like BRRE-box. Unlabeled probes were used as competitors. Experiments were repeated independently at least in triplicate with similar results. c ChIP assays showing that OsBZR4 binds to the promoters of YUC4 in vivo. Immunoprecipitation was performed with anti-Flag antibody. Immunoprecipitated chromatin was analyzed by qPCR. n = 3 biological replicates. d–e The frequency of OsBZR4p:YUC4 embryo types under SJ2 (d) and bzr4-SJ2-2 (e) background. n = 5 biological replicates, around 100 seeds per replicate. f Relative expression of PIN genes in 3-DAP caryopses. n = 3 biological replicates. g ChIP assays showing that OsBZR4 binds to the promoters of PIN5b in vivo. Immunoprecipitation was performed with anti-Flag antibody. Immunoprecipitated chromatin was analyzed by qPCR. n = 3 biological replicates. Data in (a, c–g) are presented as mean ± SD. Two-tailed unpaired Student’s t test was used for statistical analysis. Nor E normal embryo, SE small embryo, and NE no embryo in (d, e). Source data are provided as a Source Data file.
