Fig. 5: High temperature promotes embryoless phenotype of bzr4.
From: OsBZR4 regulates temperature-dependent embryogenesis in rice
a The frequency of embryo types in bzr4-SJ2-2 seeds produced at different temperatures: 22 °C (LT), 27 °C (NT), and 32 °C (HT). n = 5 biological replicates, around 100 seeds per replicate. b The frequency of embryo types in bzr4-SJ2-2 seeds produced from Harbin and Sanya. SJ2-H and bzr4-H represent plants grown in Harbin, whereas SJ2-S and bzr4-S were cultivated in Sanya. n = 5 biological replicates, around 100 seeds per replicate. c Free IAA levels in bzr4-SJ2-2 caryopses at 3 DAP under LT and HT. n = 3 biological replicates. d The relative expression level of YUC4 in bzr4-SJ2-2 caryopses at 3 DAP under LT and HT. n = 3 biological replicates. e–f The frequency of embryo types in OsPIL13 OE seeds under SJ2 (e) and bzr4-SJ2-2 (f) background, respectively. n = 5 biological replicates, around 100 seeds per replicate. g The time course expression of OsPIL13 under NT and HT. n = 3 biological replicates. h Transient transcriptional activity assay showing OsPIL13 activates YUC4 expression. Relative REN (Renilla luciferase) activity was used as an internal control, and the relative LUC (Firefly luciferase)/REN ratios are shown. n = 3 biological replicates. i The relative expression level of YUC4 in 3-DAP caryopses of SJ2 and OsPIL13 OE lines. n = 3 biological replicates. Data in (a–i) are presented as mean ± SD. Two-tailed unpaired Student’s t test was used for statistical analysis. Nor E normal embryo, SE small embryo, and NE no embryo in (a, b, e, f). Source data are provided as a Source Data file.
