Fig. 3: Structural basis for the RNA binding of ttKhpB.
From: KH–R3H domain cooperation in RNA recognition by the global RNA-binding protein KhpB
A Crystal structure of the RNA-bound form of the ttKhpB dimer. ttKhpB and RNA are shown in the cartoon and stick models, respectively. The KH and R3H domains of each subunit are color-coded as in Fig. 1D. The residues in the observed 7 nucleotides of the RNA are labeled from C1 to C7 in the direction from the 5’ to 3’ end. B Surface representation of the RNA-bound form. The same structure as in (A) is shown with ttKhpB in a sphere model, highlighting the RNA-binding groove. C Electrostatic surface was calculated using the Adaptive Poisson-Boltzmann Solver program and visualized with PyMOL. A positively charged surface patch (in blue) is observed at the RNA-binding site.
