Fig. 1: Disrupted planar cell polarity in the neuroectoderm of FGFR1-depleted embryos.

A Experimental scheme. Adapted from Xenopus illustrations © Natalya Zahn (2022), via Xenbase (www.xenbase.org, RRID: SCR_003280). Thirty-two-cell Xenopus embryos were co-injected with 5 nl of control (Co) or FGFR1 morpholino (MO), 15 ng each, along with GFP RNA (90 pg) as a lineage tracer into one dorsal animal blastomere. Embryos were collected at stage 15 (st.15) and co-immunostained for Vangl2 (red) and GFP (green). The anteroposterior (AP) axis is indicated. Representative en face neural plate images are shown in (B) and (C), white box areas are magnified on the right to show merged (green+red) and single (red) channel images. B Control neuroectoderm with anteriorly polarized Vangl2 (arrows). C FGFR1MO-injected neuroectoderm lacking anterior Vangl2 accumulation (asterisks). D Quantification of GFP-positive cells with anteriorly enriched Vangl2 in CoMO- and FGFR1MO- injected neuroectoderm. Data are presented as a dot plot with horizontal lines indicating the mean. Each dot represents the percentage of polarized cells per embryo, with three embryos per group and 50–90 cells scored per embryo. Total numbers of cells (n) analyzed per group are indicated above each condition. Data are representative of four independent experiments, p = 0.00074, two-tailed unpaired Student’s t-test. Scale bar, 30 µm.