Fig. 7: Perturbation of both the trimer and lateral structures impair nervous system function and neurotransmitter secretion.
A Animals will spontaneously swim or “thrash” rhythmically when transferred to a fluid medium. This form of locomotion requires a functional nervous system. B Average thrashing frequency for unc-13(s69) (orange, n = 43) or unc-13(s69) expressing a transgene encoding wild-type UNC-13L (gray, n = 39), UNC-13L(2xR) (red, n = 60), 2xD (blue, n = 53), 3xD (green), IL/NN (purple, n = 62), or 2xE (cyan, n = 46). C Cartoon depicting a C. elegans neuromuscular junction secreting ACh (red). Synaptic cleft acetylcholinesterase (green) hydrolyzes ACh and this process is blocked in the presence of aldicarb. D Paralysis time course on 1 mM aldicarb for wild type (black) and unc-13(s69) mutants expressing wild-type UNC-13L (gray), 2xR (red), or IL/NN (blue). Data were collected from N = 11, 9, 10, and 13 biological replicates, respectively. Each replicate comprised ~20 animals. Data were mean ± SEM. For statistical comparisons, data were compared using one-way ANOVA and Tukey–Kramer analysis for multiple comparisons. **p < 0.01 and * p < 0.05 compared to wild type. In B, the p values for wild type versus unc-13(s69), 2xR, 2xD, and IL/NN were <1 × 10−14. For wild type versus 3xD and 2xE, the p values are 1.6 × 10−11 and 1.5 × 10−8, respectively. See Methods for the individual p values in (D).
