Fig. 3: SC35-mediated splicing of bZIP49 enhances salt sensitivity in bZIP49Lox plants.
From: SC35-mediated bZIP49 splicing regulates K⁺ channel AKT1 for salt stress adaptation in poplar
a GO enrichment analysis of positive yeast clones (hypergeometric test). Bubbles size reflects enrichment factor. b RNA-EMSA indicated the binding of GST-SC35 to the “GGAAUUAG” RNA motif. c, d RT-qPCR of bZIP49L/S transcripts (c) and splicing index (d) in bZIP49Lox, SC35ox/bZIP49Lox, and sc35cr/bZIP49Lox plants. Data represent mean ± SE (n = 3 technically replicates; ns P > 0.05; **P < 0.01; ***P < 0.001; one-way ANOVA). e Immunoblot analysis of FLAG-bZIP49L following immunoprecipitation in bZIP49Lox and SC35ox/bZIP49Lox plants. f Phenotypes comparison of 60-day-old transgenic lines under normal and 100 mM NaCl conditions. g, h K+ (g) and Na+ content (h) of different genotypes under normal and salt stress conditions. Box plots show medians, interquartile ranges, and 1.5 × IQR whiskers; dots indicate outliers (n = 12 biological replicates). i, j Net K+ (i) and Na+ (j) fluxes in root meristem and elongation zone. Bar charts show the mean values across the time course and replicates. Values are means ± SE (n = 3 biological replicates). Different letters indicate significant differences (P < 0.05, two-way ANOVA; P values are shown in Source Data file). Source data are provided as a Source Data file.
