Fig. 4: Transcriptome analysis shows that bZIP49S may inhibits AKT1.
From: SC35-mediated bZIP49 splicing regulates K⁺ channel AKT1 for salt stress adaptation in poplar
a Venn diagram shows overlapping genes from two groups of differentially expressed genes. b GO enrichment analysis was performed on the 5491 overlapping genes using the hypergeometric test with the ClusterProfiler R package, identifying significantly overrepresented biological processes. The top 20 GO terms are shown, with the y-axis representing biological processes and the x-axis representing enrichment significance. Bubble size indicates the enrichment factor, the ratio of upregulated genes in a biological process to all genes annotated in that process. c EMSA showing bZIP49 binding to the AKT1 promoter. Biotin-labeled probes were used for protein-DNA binding, with unlabeled probes as competitors. +, added, ++, added at twice the volume. Three biological replicates were performed with similar results. d The effect of bZIP49 on AKT1 expression was analysis using an effector/reporter-based gene transactivation assay in N. benthamiana leaves, with a schematic illustrating the effector and reporter structures. e Luciferase activity analysis. Data are the means ± SE; n = 3 biological replicates (ns, P > 0.05; ***, P < 0.001, one-way ANOVA). Source data are provided as a Source Data file.
