Fig. 2: Single nucleus transcriptional profiles of mouse brainstems. | Nature Communications

Fig. 2: Single nucleus transcriptional profiles of mouse brainstems.

From: Scn2a-linked myelination deficits and synaptic plasticity alterations drive auditory processing disorders in an ASD mouse model

Fig. 2

A (Top) UMAP plots of snRNA-seq dataset with clusters identified as OL lineage cells, indicated by dashed line circles; OPC, Differentiating OL1 (Diff. OL1), Differentiating OL2 (Diff. OL2), Premature OL, and Mature OL. (Bottom) Expression pattern of OL lineage marker genes such as Pdgfra and Mbp. Graded color indicators showed the scaled gene expression level. B (Left) The distribution of single cells colored according to the five OL lineage states identified by trajectory analysis from OPCs to mature OLs (gray line). (Right) The percentage (%) of each cluster (from OPC to Mature OL) in total OLs from individual controls (n = 2) and cKO mice (n = 2). C (Left) UMAP plots of snRNA-seq for OL lineage cells from control (blue dots) and cKO (red dots). (Right) The number of DEGs for each OL lineage. D Volcano plot of differentially expressed myelin related genes (391 genes) in control and cKO mice (without correction for multiple comparison). E 33 genes showed significant differential expression (p < 0.05, without correction for multiple comparison) and interconnection between those genes was detected in String analysis. Those genes were related to myelin structure (green line), cell junction (red), membrane protein signaling (blue), and ion channel (yellow). F Violin plots of top eight genes being downregulated in Scn2a cKO mice: Mobp, Ncam1, Ptn, Gnao1, Mtmr2, Abca2, Arhgef10 and Mpdz.

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