Fig. 4: SUMOylation of PABPC1 promotes mRNA stability under stress.
a Flowchart of the 4sU pulse-chase sequencing (4sU-Seq) process. Cumulative distribution plot (b) and frequency distribution plot (c) of mRNA half-life in H1299PABPC1–/– cells re-expressing Myc-PABPC1-WT/K512R under normal condition. Cumulative distribution plot (d) and frequency distribution plot (e) of mRNA half-life in H1299PABPC1–/– cells re-expressing Myc-PABPC1-WT/K512R under AS-mediated stress condition. f KEGG pathway analysis of genes in the 4sU-Seq data that showing a twofold increase in half-life in H1299PABPC1–/– cells re-expressing Myc-PABPC1-WT under stress condition, P-values were calculated by DAVID bioinformatics tools using Fisher’s Exact Test. g List of enriched genes in the Mitophagy pathway. h, i 4sU-RT-qPCR was performed to detect the stability of FUNDC1, BECN1, and BNIP3L mRNA in H1299PABPC1–/– cells re-expressing Myc-PABPC1-WT/K512R under normal and stress conditions. Decay graphs were generated by applying the one-phase decay model. For Cumulative fraction analysis, P-values were calculated using a two-sided Mann–Whitney U test (b, d). For 4sU-RT-qPCR, data were presented as mean ± SD, n = 3 biologically independent replicates, P-values were determined by one-side sum-of-squares F test (h–j).
