Fig. 2: CSLD5 regulates cell growth and division at the SAM.

a Comparison of cell size in wild-type (WT, Col-0) and csld5 SAMs. The L1 (epidermal) cells were segmented in MorphoGraphX and shown as a heatmap. Scale bar, 50 µm. b Reduced cell division activity in csld5. Cells division was tracked using the plasma membrane marker myr-YFP (shown in blue). The cells that divide within a 24-h interval are labeled in magenta. Data represent means ± SD. n = 6 SAMs for WT and 6 SAMs for csld5. Scale bar, 50 µm. c Comparison of new cell wall morphology in wild-type (WT, Col-0), csld5, and csld2 csld5 SAMs. Left panels show the segmented L1 (epidermal) cells, with red lines labeling the newly formed cell walls within 24 h (for WT), 24 h (for csld5), or 24 and 72 h (for csld2 csld5). The projected cells are shown in the right panels, with the new walls indicated by stars. Note that there were no cell division in the csld2 csld5 double mutant in the first 24 h. Scale bar, 10 µm. d Analysis of cell growth in wild-type and csld5 SAMs. Heatmaps show the relative cell size increase over 12 h in the L1 cells, with a value of 1 indicating no growth. The growth rate of csld5 cells is reduced compared to that of wild-type cells. Scale bar, 50 µm. Boxplots (a, d) indicate median (box center line), 25, 75% (box), and the whiskers extend to the minima and maxima. ^****P < 0.0001 (Unpaired two-tailed Student’s t-test). Source data are provided as a Source Data file.