Fig. 3: The chemical distribution maps of the GP-epiphysis interface and GP-metaphysis interface revealed by Raman microscopic detection technology.

A, C Raman spectra collected in the 300 ~ 1300 cm⁻¹ from GP to (A) epiphysis and (C) metaphysis at different area (zone 1–6) in (B) and (D), The information of marked Raman shifts: PO₄³⁻ v₁ band at 950 ~ 960 cm⁻¹, PO₄³⁻ v₂ band at 430 ~ 433 cm⁻¹ and PO₄³⁻ v₄ band at 573 ~ 590 cm⁻¹ and CO₃^2–v₁ band at 1071 cm^–1; B, D The maps of ACP (950 cm^-1), ACP/HAp interfering peaks (955 cm^–1), HAp (960 cm^–1) and GAG (1410 cm^–1) in B) GP-epiphysis interface and D) GP-metaphysis interface by SRS imaging, scale bar = 10 μm; E, H Raman peak intensity maps of PO₄^3– v₁ band at 960 cm^–1 and corresponding maps of full-widths at half-maximum (FWHM) of the peak at 960 cm^-1 at (E) GP-epiphysis interface and (H) GP-metaphysis interface, scale bar = 5 μm; F, I The maps of CO₃^2– v₁ band at 1071 cm^–1 and CO₃^2–/ PO₄^3– ratio at (F) GP-epiphysis interface and (I) GP-metaphysis interface, revealing the ionic substitution of minerals in both interfaces, scale bar = 5 μm; G, J The mineral to collagen maps (960 cm^–1 to 1595 ~ 1700 cm^–1) of G) GP-epiphysis interface and (J) GP-metaphysis interface, revealing relative content and distribution of HAp, scale bar = 5 μm. Source data are provided as a Source Data file.