Fig. 10: The PCAF/SIRT7-mediatded IDH1 Kcr exhibits progressive suppression in parallel with advancement of MASLD.

a Representative images of liver tissues from healthy controls, MASLD and MASH patients were presented, including H&E-stained sections, Oil Red O staining, Masson’s trichrome staining and Sirius red staining. Scale bars: 100 μm. b Protein lysates from the liver tissues from healthy, MASLD and MASH patients were extracted for western blotting using IDH1-K58cr, IDH1-K151cr, IDH1-K212cr, IDH-K345cr, IDH1, PCAF, SIRT7, α-SMA and α-Tubulin antibodies. c Relative mRNA expression levels of PCAF and SIRT7 in liver tissues from healthy and MASLD patients (n = 16/group). d Hepatic PCAF and SIRT7 levels were associated to MASLD progression in two independent cohorts (GSE135251, GSE130970). e Schematic outline illustrates the mechanisms that the PCAF/SIRT7-mediatded IDH1 Kcr alleviates MASLD progression by enhancing the TCA cycle. Created in BioRender. Liu, S. (2025) https://BioRender.com/rxqskmj. Data are shown as the mean ± SD. P values were obtained using two-tailed unpaired Student’s t-test. All data were verified in three independent experiments. Source data are provided as a Source Data file.