Fig. 6: IDH1 crotonylation attenuates lipid deposition in AML12 cells.

a AML12 cells were treated with control or IDH1 siRNA for 24 h, followed by expression of Myc-tagged Vector, IDH1-WT, IDH1-K58R, IDH1-K151R, IDH1-K212R, IDH1-K345R, IDH1-4KR and IDH1-4KQ. After another 24 h, cells were treated with BSA (top) or 0.5 mM PA + OA (bottom) for 24 h, then fixed and stained with Oil Red O. Scale bars: 100 μm. b Quantification of the Oil Red O staining area as described in (a) (n = 6/group). c The expression of the indicated proteins was measured in AML12 cells as described in (a). d AML12 cells were treated with control or PCAF siRNA for 48 h before cells were treated with BSA (top) or 0.5 mM PA + OA (bottom) for another 24 h, then fixed and stained with Oil Red O. Scale bars: 100 μm; e Quantification of the Oil Red O staining area as described in (d) (n = 3/group). f The expression of the indicated proteins was measured in AML12 cells as described in (d). g FLAG-tagged SIRT7 were expressed in AML12 cells for 48 h, followed by treatment with BSA (top) or 0.5 mM PA + OA (bottom) for 24 h, fixed and stained with Oil Red O. Scale bars: 100 μm. h Quantification of the Oil Red O staining area as in (g) (n = 3/group). i The expression of the indicated proteins was measured in AML12 cells as described in (g). Data are shown as the mean ± SD. P values were obtained using one-way ANOVA followed by Tukey’s multiple comparisons test (b) or two-tailed unpaired Student’s t-test (e, h). All experimental data were verified at least three independent experiments. Source data are provided as a Source Data file.