Fig. 4: Structural basis of hSLC15A4 state transitions.
From: Development of conformation-selective antibodies targeting human SLC15A4
Rocking motions of NTD (a) and CTD (b) of hSLC15A4 (luminal-open state: gray, cytoplasmic-open state: palegreen and lightblue) after structural alignment of both transporter units. Red lines show the shift of the TMs. c Structural superimposition of hSLC15A4-Fab107 complex (colored in lightpink) and hSLC15A4-TASL-Fab235 complex (colored in palecyan) in lateral view (left) and lysosomal lumen view (right). Lateral view (d), endolysosome lumen view (e), and cytoplasmic view (f) of hSLC15A4 in luminal-open state. g NTD-centered structural superimposition of cytoplasmic-open hSLC15A4 (colored in palegreen and lightblue) and luminal-open hSLC15A4 (colored in gray). Black arrows indicate the oscillation of the CTD. Lateral view (h), endolysosome lumen view (i), and cytoplasmic view (j) of hSLC15A4 in cytoplasmic-open state.
