Fig. 2: CYP1B1-AS1 and ENSG00000286147 (lnc-DKK2) as C.burnetii infection-associated lncRNAs.
a Hierarchical clustering heatmap of differentially expressed (DE) lncRNA-mRNA pairs during C. burnetii (Cb, NMII) infection. Each row represents a gene, and each column represents a biological replicate (n = 4). Color intensity indicates normalized gene expression. b RT-qPCR validation of DE lncRNAs specific to Cb and CbΔdotA (CbA) infections. Gene expression normalized to ACTB and shown relative to mock-infected controls (set to 1). Data represent the mean ± SD from three independent experiments (n = 3). Statistical test: two-way ANOVA; exact p-values (in the same order as the asterisks): lnc-DKK2: 0.035; PKP4-AS1: 0.008; GSTCD-AS1: 0.0487; DDX11-AS1: 0.0432; ENSG00000285650: 0.045; ENSG00000261668: 0.008; ENSG00000273669: 0.0087; ENSG00000260430: 0.035; LBX2-AS1: 0.0279; LINC03072: 0.008; TLR8-AS1: <0.0001; LINC01232: 0.0002; SBF2-AS1: <0.0001; LINC00942: 0.002; LINC00926: 0.008; LINC01426: 0.004; NRAD1: 0.0326; UBR5-DT: 0.0086; PIRAT1: 0.0049; CYP1B1-AS1: <0.0001. c Scatter plot of DE lncRNAs and mRNAs during Cb infection, highlighting CYP1B1-AS1 (violet), lnc-DKK2 (blue), and their target mRNAs CYP1B1 (green) and DKK2 (yellow). d Receiver operating characteristic (ROC) curve analysis displaying the diagnostic value of lnc-DKK2 and CYP1B1-AS1 in infection models, with area under the curve (AUC) values at p < 0.01 and 95% confidence intervals (CI). e, f KEGG pathway enrichment analysis of mRNAs associated with DE lncRNAs: e upregulated, f downregulated. Statistical test: one-sided Fisher’s exact test; p-values adjusted using the Benjamini–Hochberg false discovery rate (FDR) method. Bar height represents −log10(p-value) with color intensity representing the proportion of transcripts associated with each pathway. Source data are provided in the Source Data file.
