Fig. 3: Spatiotemporal expression of CYP1B1-AS1 and lnc-DKK2 during C.burnetii infection.

a RT-qPCR analysis of CYP1B1-AS1 and CYP1B1 in infected THP-1 macrophages (NMII) at the indicated time points. Statistical test: two-way ANOVA; exact p-values (in the order as asterisks): CYP1B1-AS1: 0.03, 0.009, 0.003, 0.0038, and 0.0044; CYP1B1: 0.021, 0.0098, 0.009, 0.0042, and 0.0086. b RT-qPCR analysis of CYP1B1-AS1 and CYP1B1 in infected HeLa cells. Statistical test: two-way ANOVA; exact p-values (in the order as asterisks): CYP1B1-AS1: 0.0089, 0.05, 0.0035, 0.048, and 0.036; CYP1B1: 0.029, 0.0395, 0.0027, 0.0102, and 0.033. c, d RT-qPCR analysis of lnc-DKK2 and DKK2 in infected c THP-1 macrophages and d HeLa. Statistical test: two-way ANOVA; *p < 0.05; **p < 0.01; ns, not significant, p ≥ 0.05. Exact p-values are provided in the Source data file. For a–d, expression was normalized to ACTB and shown relative to mock-infected controls (set to 1). Data represent mean ± SD from three independent experiments. e, f Bulk RNA-seq–based tissue expression profiles of e CYP1B1-AS1 and f CYP1B1 from the Genotype-Tissue Expression (GTEx v.10) database, shown as log₁₀-transcripts per million (TPM + 1). TPMs were computed from gene models with isoforms collapsed to a single gene; no additional normalization was applied. These data are derived from a publicly available RNA-seq database; biological/technical replication is not applicable. Box plots show the median (center line), 25th and 75th percentiles (box), and whiskers at 1.5× the interquartile range (IQR). Data points outside this range are plotted as individual outliers. Tissues include brain regions (e.g., amygdala, hippocampus, cerebellum) and peripheral tissues (e.g., liver, lung, kidney, spleen, blood).