Fig. 4: Temporal analysis of CYP1B1-AS1 and immune-responsive lncRNAs in primary human monocyte-derived macrophages (hMDMs) during C. burnetii NMI infection.

hMDMs were either infected with Cb-NMI at an MOI of 10 or stimulated with lipopolysaccharide (LPS, 10 ng/mL). RT-qPCR analysis of (a) CYP1B1-AS1 and CYP1B1 and (b) PIRAT1 and LUCAT1 in Cb-NMI-infected hMDMs at various time points (p.i.). Statistical test: two-way ANOVA; exact p-values (in the same order as asterisks): CYP1B1-AS1: 0.0075, 0.0218, 0.0089; CYP1B1: 0.0006, 0.0458, 0.0057; PIRAT1: 0.001, 0.0037, 0.0029; LUCAT1: 0.001, 0.0035, 0.014, 0.0229; and ns, not significant, p ≥ 0.05. RT-qPCR analysis of (c) CYP1B1-AS1 and CYP1B1, d PIRAT1 and LUCAT1 in LPS-treated hMDMs. Statistical test: two-way ANOVA; exact p-values (in the same order as asterisks): CYP1B1-AS1: 0.0006, 0.0008 and 0.0178; CYP1B1: 0.0039, 0.0002, 0.0003 and 0.0122; PIRAT1: 0.0007 and 0.0051; LUCAT1: 0.0022, 0.0007, and 0.05; and ns, p ≥ 0.05. Expression dynamics of core immune-responsive lncRNAs (MAILR, LINC01215, PACER, MROCKI, MIR155HG, and MIR222HG) in response to (e) Cb-NMI infection or (f) LPS stimulation. Statistical test: two-way ANOVA; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns not significant, p ≥ 0.05. Exact p-values are provided in the Source data file. For all data a–f: expression normalized to ACTB and shown as fold changes relative to uninfected or unstimulated controls (mock, set to 1). Data represent mean ± SD of three technical replicates from two independent experiments. g GTEx-heatmap showing co-expression of CYP1B1-AS1, CYP1B1, and AHR across human tissues. Values are presented as log₁₀(TPM + 1) with color intensity representing relative gene expression.