Fig. 8: Knockdown of CYP1B1-AS1 and CYP1B1 induces mitochondrial ROS and impairs mitochondrial function. | Nature Communications

Fig. 8: Knockdown of CYP1B1-AS1 and CYP1B1 induces mitochondrial ROS and impairs mitochondrial function.

From: CYP1B1-AS1 regulates CYP1B1 to promote Coxiella burnetii pathogenesis by inhibiting ROS and host cell death

Fig. 8: Knockdown of CYP1B1-AS1 and CYP1B1 induces mitochondrial ROS and impairs mitochondrial function.

Flow cytometry and metabolic assays were performed on THP-1 macrophages with siRNAs targeting CYP1B1 (CYPB), CYP1B1-AS1 (lncCYPB), or both (dCYPB), and C. burnetii-infected (NMII) conditions at 24 h p.i. a, b Total ROS production assessed by flow cytometry, measured as mean fluorescence intensity (MFI) of DCFDA-stained cells compared to mock. Statistical test: one-way ANOVA; ****p < 0.0001; **p = 0.006 (NMII); **p = 0.0021 (NC: NMII). c, d Mitochondrial ROS production assessed by flow cytometry, measured as MFI of MitoSOX™ Red-stained cells. e, f Mitochondrial membrane potential assessed by flow cytometry, measured as MFI of MitoProbe™ TMRM-stained cells. For d, f; Statistical test: one-way ANOVA; ****p < 0.0001. g Oxygen consumption rate (OCR) measured using an Agilent Seahorse Metabolic Analyzer to evaluate mitochondrial respiration. h Maximal respiration was measured across knockdown and controls. Statistical test: two-way ANOVA; ****p < 0.0001. All data represent mean ± SD from three independent experiments (n = 3). Source data are provided as a Source Data file.

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