Fig. 6: Incorporating cytoskeletal feedback into a core model of the STEN.

a Schematic showing the signal transduction network involving Ras/ PIP2 and PKB and how they couple to the two types of actin feedback loops. b Kymographs showing activity around the cell perimeter as a function of time. PIP2 and Ras are shown in green and magenta, respectively. The top and bottom correspond to simulations without and with the feedback loops, respectively. c Simulated cell trajectories of 10 cells each with feedback loops turned off (top) and on (bottom). d Kymographs showing wave activity across the cell perimeter for varying strengths of the branched actin feedback. e Total Ras activity around the cell perimeter with respect to the strength of the branched actin feedback. Wildtype corresponds to a strength of 1. The black bar denotes the mean of 10 simulations per strength. Simulations with total Ras activity less than 500 showed no firings. f Kymographs showing wave activity across the cell perimeter for varying strengths of the actomyosin feedback. g Total Ras activity around the cell perimeter with respect to the strength of the actomyosin feedback. Wildtype corresponds to a strength of 0.4. The black bar denotes the mean of 10 simulations per strength. Simulations with total Ras activity less than 200 showed no firings. h Frames from a 2D simulation of the effects of adding CK666 and then Latrunculin to wild-type cells. The three rows represent waves in the wild-type cell, waves after CK666 addition, and subsequent Latrunculin treatment. i The total Ras activity for simulations as in h. The solid line and the shaded area represent the mean ± 1 standard deviation. In all the simulations, the CK666 effect is incorporated at the 200 s and the additional Latrunculin effect is added at 400 s. Scale bars = 10 microns. Source data are provided as a Source Data file.