Fig. 7: The STEN model incorporating cytoskeletal feedback predicts changes to motility from modifying branched actin and actomyosin.

a, b 10 simulated tracks of cell motion before (left) and after (right) increasing the strength of the branched actin loop (a) or decreasing the strength of the actomyosin loop (b). Cells begin with low feedback strength for both branched actin and actomyosin, creating low polarity. 10 simulated tracks of cell motion before (left) and after (right) increasing the strength of the branched actin loop (c) or decreasing the strength of the actomyosin loop (d). Cells begin with high feedback strength for both branched actin and actomyosin, creating high polarity. e Tracks of vegetative AX3 and abnABC⁻ cells migrating for 10 minutes. f Tracks of vegetative AX3 cells expressing mCherry-FRB-MHCKC and an unlabeled membrane-localized FKBP domain (cAR1-2xFKBP) migrating for 10 minutes before rapamycin addition to recruit MHCKC and for 10 minutes after 10 minutes of incubation in rapamycin. g Tracks of developed, polarized AX3 and abnABC⁻ cells migrating for 10 minutes. h Tracks of developed, polarized AX3 cells expressing mCherry-FRB-MHCKC and an unlabeled membrane-localized FKBP domain (cAR1-2xFKBP) migrating for 10 minutes without rapamycin or after 10 minutes of incubation in rapamycin. i Average (lines) and individual (dots) estimated 2D diffusion coefficients of cells in each indicated condition from E-F. All cells are vegetative. j Average and individual estimated 2D diffusion coefficients of cells in each indicated condition from G-H. All cells are developed. *=p = 0.041, **=p = 0.0057, ***=p < 0.005, 2-sided Mann-Whitney U test. Source data are provided as a Source Data file.