Fig. 2: Effect of βCD treatment on the structure of YnaI in DOPC/MSP2N2 nanodiscs and on the function of YnaI in soy polar liposomes. | Nature Communications

Fig. 2: Effect of βCD treatment on the structure of YnaI in DOPC/MSP2N2 nanodiscs and on the function of YnaI in soy polar liposomes.

From: Lipid interactions and gating hysteresis suggest a physiological role for mechanosensitive channel YnaI

Fig. 2

a The overlay of the structure of YnaI in DOPC nanodiscs before (one subunit shown as cyan ribbons) and after treatment with βCD (one subunit shown as cornflower blue ribbons and the other six subunits as white ribbons) shows that nanodisc-embedded YnaI remains in the closed conformation upon treatment with βCD. b Representative patch-clamp electrophysiology recordings (from sets of 5 recordings each) of soy polar liposomes reconstituted without (top panel) and with YnaI (bottom panel) that were treated with 10 mM βCD. The pipette potential was +30 mV. The increase in current shortly before rupture of the YnaI-containing liposomes shows that βCD-mediated lipid removal can activate YnaI in liposomes. c Quantification of peak currents from 5 recordings each that were induced by βCD treatment of soy polar liposomes without (Empty) and with YnaI. The box-and-whisker plot shows the median current (line), the upper and lower quartiles (box), and the upper and lower extremes (whiskers).

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