Fig. 1: Global knockout of RKIP inhibits bone resorption and promotes bone formation. | Nature Communications

Fig. 1: Global knockout of RKIP inhibits bone resorption and promotes bone formation.

From: RKIP regulates bone marrow macrophage differentiation to mediate osteoclastogenesis and H-type vessel formation

Fig. 1: Global knockout of RKIP inhibits bone resorption and promotes bone formation.

A Micro-CT and three-dimensional (3D) reconstruction images of the distal femur of 3-month-old male RKIP+/+ or RKIP-/- mice are shown. B Quantification of bone volume/tissue volume (BV/TV), trabecular number (Tb.N), trabecular separation (Tb.Sp), trabecular thickness (Tb.Th), and cortical thickness (Ct.Th) (n = 5). C H&E staining and TRAP staining of 3-month-old male RKIP+/+ or RKIP-/- mice femurs sections. D Quantification of osteoclast number per bone surface (N.Oc/BS) and osteoclast surface per bone surface (Oc.S/BS) (n = 6). E Calcein double staining and Von Kossa staining of 3-month-old male RKIP+/+ or RKIP-/- mice femurs sections. F Quantification of mineral apposition rate (MAR) and bone formation rate per bone surface (BFR/BS) (n = 6). G OCN (green) and DAPI (blue) immunofluorescence of 3-month-old male RKIP+/+ or RKIP-/- mice femurs sections; The arrow indicates the cells which express OCN. H Quantification of OCN+ cell number per bone perimeter (N.OCN+/B.Pm) (n = 6). I Serum C-terminal telopeptide of type I collagen (CTX-1) and Procollagen I N-Terminal Propeptide (PINP) concentration measured by ELISA from RKIP+/+ or RKIP-/- male mice (n = 6). Data are presented as mean ± SD. *P < 0.05, **P < 0.01 for a comparison with the control group or as indicated. n value means the number of repetitions in each independent experiment.

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