Fig. 3: RKIP enhances osteoclastogenesis by interacting with ARHGAP to suppress CDC42 inactivation. | Nature Communications

Fig. 3: RKIP enhances osteoclastogenesis by interacting with ARHGAP to suppress CDC42 inactivation.

From: RKIP regulates bone marrow macrophage differentiation to mediate osteoclastogenesis and H-type vessel formation

Fig. 3: RKIP enhances osteoclastogenesis by interacting with ARHGAP to suppress CDC42 inactivation.

A GO enrichment analysis of RNA-seq analysis of BMMs from RKIP+/+ and RKIP-/- mice. B CDC42 peptides identified through mass spectrometry are shown. C Western blots of CDC42-GTP and total CDC42 protein levels in BMMs from RKIP+/+ or RKIP-/- male mice with RANKL treatment for indicated time. D RKIP (green), CDC42 (red) and DAPI (blue) immunofluorescence staining of BMMs from RKIP+/+ or RKIP-/- male mice with or without RANKL treatment. E Various truncated fragments of RKIP (R1, 1–90; R2, 91–140; R3, 141–187). R1–R3 and full length of CDC42 plasmid were co-transfected in HEK-293T cells. The interactions were detected through the indicated IP and IB analyses. F Various truncated fragments of CDC42 (C1, 1–47; C2, 48–104; C3, 105–191). C1–C3 and full length of RKIP plasmid were co-transfected in HEK-293T cells. The interactions were detected through the indicated IP and IB analyses. G Overall structure of CDC42 (pink) and RKIP (purple) and key residues of their interaction are shown. H HEK-293T cells were transfected with CDC42-Flag or CDC42-Flag and RKIP-Myc; The interactions were detected through the indicated IP and IB analyses. I HEK-293T cells were transfected with ARHGAP-HA and CDC42-Flag, or ARHGAP-HA, CDC42-Flag and RKIP-Myc; The interactions were detected through the indicated IP and IB analyses. J HEK-293T cells were transfected with RKIP-Myc, ARHGAP-HA or RKIP-Myc and ARHGAP-HA; The interactions were detected through the indicated IP and IB analyses. K The illustration of RKIP inhibiting CDC42 inactivation by interacting with ARHGAP.

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