Fig. 4: GAF is mitotically retained at key differentiation gene loci that exhibit increased H3K27ac levels in neural progenitors. | Nature Communications

Fig. 4: GAF is mitotically retained at key differentiation gene loci that exhibit increased H3K27ac levels in neural progenitors.

From: Dual mitotic bookmarking by GAF and H3K27ac orchestrates differential propagation of cell fate memory in neural development

Fig. 4: GAF is mitotically retained at key differentiation gene loci that exhibit increased H3K27ac levels in neural progenitors.

a Experimental workflow of mitotic and interphase NSC sorting in a drug synchronization-free manner followed by CUT&Tag analysis. Created in BioRender. Li, Y. (2025) https://BioRender.com/pcljp15. b Genome browser examples of CUT&Tag for GAF tracks at key differentiation gene loci in interphase and mitotic NSCs. Representative examples of GAF binding peaks (mitotically retained, interphase only, and mitosis only) are marked by gray boxes. c Venn diagram showing GAF interphase only, mitotically retained, and mitosis only peaks. d A schematic drawing depicting the asymmetric division of NSC produces sibling daughter cells with distinct cell fates. e Genome browser images of CUT&Tag for GAF tracks at key differentiation gene loci in NSCs versus progenitors. Decreased NSC-specific binding peaks of GAF in progenitors are marked by gray boxes. f Genome browser images of CUT&Tag tracks for H3K27ac, H3K14ac, and H3K4me at key differentiation gene loci in NSCs versus progenitors. Increased H3K27ac binding peaks at key differentiation gene loci in progenitors in comparison to NSCs are marked by gray boxes. Here pnt and tub gene loci serve as negative controls.

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