Fig. 3: Interactions between the spKA-1 domain and spISL.
From: Structures and mechanisms of U6 snRNA m6A modification by METTL16
a–d Detailed views of the interactions between spKA-1 and spISL. The colors for nucleosides are the same as in Fig. 2d. e In vitro methylation of the spU6 snRNA transcript by spMETTL16 and its variants with mutations in the KA-1 domain under standard conditions. spU6 snRNA (0.5 μM) was incubated with 200 nM spMETTL16 or its variants in the presence of 1 mM SAM for 4 min at 37 °C. Error bars represent the standard deviation (SD) of three independent experiments (N = 3), and the center of the error bands indicates the mean of the measured values. Source data are provided as a Source data file. Semi-quantitative RT-PCR analyses of two introns: f SPAC18B11.09 C and g ckn1. The upper and lower bands on the gel represent retained and spliced introns, respectively. Intron retention in the Δmtl16 strain was rescued by ectopic expression of plasmid-encoded full-length METTL16 (spMETTL16), but not by the spMETTL16 variants with mutations in the KA-1 domain shown in (e) (Supplementary Fig. 2c, d).
