Fig. 5: Interactions between spMETTL16 and spU6 snRNA in the spMETTL16–SAM–spU6 complex.
From: Structures and mechanisms of U6 snRNA m6A modification by METTL16
a Overall structure of spMETTL16–SAM–spU6 (left) and nucleotide sequence of spU6 snRNA. The modeled nucleotides (35–83) in the structure are colored orange. b SAM recognition by spMETTL16. The structure of human METTL16 MTD in complex with SAH (hsMETTL16-SAH)41 was superimposed onto that of spMETTL16–SAM–spU6. SAH in the hsMETTL16-SAH is shown as yellow sticks and SAM in the spMETTL16–SAM–spU6 is shown as cyan sticks. c, d Interactions between spMETTL16 and spU6 snRNA. e Schematic representation of the interaction between A35C36A37G38A39 and the MTD in (c, d). f In vitro methylation of the spU6 snRNA transcript by spMETTL16 and its variants with mutations in the KA-1 domain under standard conditions. spU6 snRNA (0.5 μM) was incubated with 200 nM spMETTL16 or its variants in the presence of 1 mM SAM for 4 min at 37 °C. Error bars represent the standard deviation (SD) of three independent experiments (N = 3), and the center of the error bands indicates the mean of the measured values. Source data are provided as a Source data file.
